Essay --

deoxyribonucleic acid isolation and expansion of ansA1 and ansA3 genesGood quality genomic DNA was isolated (Sambrook, et al 2002) and bothansA1 and ansA3 genes were amplified by PCR. lay down bands of both the genes showing a size of 1kb were observed down the stairs UV transilluminator after agarose gel electrophoresis (Fig 1).Figure 1 PCR amplification of both ansA1 and ansA3 genes way (A) Step up 100bp marker, Lane (B) amplification of ansA1 gene, Lane (C) amplification of ansA3 geneOverexpression and purificationThe overexpressed recombinant proteins (rBliAI and rBliAIII) of B. licheniformis MTCC 429 were purified to homogeneity by affinity chromatography using Ni-NTA column. Overexpression of recombinant His-tagged asparaginase into E.coli leads to improvement in yield and affinity purification process of enzyme (Enriquez et al, 2012). The fractions showing figurehead of protein with the help of Bradfords reagent were pooled together. The pooled protein solution was dialyz ed against the same buffer and was checked for the L-asparaginase activity.The purified protein after SDS PAGE or Native PAGE showed a single band (Fig 2) illustrating its similar nature. The molecular weight of the subunits of ansA1 and ansA3was found to be approximately 37kD after SDS PAGE abbreviation. SDS PAGE analysis of recombinant L-asparaginase from Pyrococcus furiosus displayed single 37kDa band (Bansal et al, 2010). In another study, recombinant ansA from Rhizobium etliin SDS-PAGE showed the comportment of a single polypeptide chain of 47kDa (Enriquez et al, 2012).Native PAGE analysis showed the molecular weight of the purified protein as 74 kDa. This study confirmed that the ansA1 and ansA3 enzymes from Bacillus licheniformis MTCC 429 are a homodimer in... ...81.50x106Roth et al, 2013 prorogue Comparative kinetic parameters for hydrolysis of L-asparagineThe kinetic properties of recombinant BliA were investigated and the parameters like kcat and kcat/Km were also stud ied with L-asparagine as a substrate. Comparative studies of the rBliA with asparaginases from various microbial sources showed that rBliA has lower Km measure for L-asparagine (Table 2) confirming thatrBliA hasbetter affinity towards its substrate than other reported (Table) microbial sources. The catalytic constant (kcat) of rBliA was completely 1.5 times high than the L-asparaginase from E.coli and catalytic constant of rErAII was 1.35 times higher than rBliA. The absolute value of kcat/Km suggests the catalytic efficiency of the enzyme (Price and Nairn, 2009). kcat/Km of the rBliAIII was 5.4x 104 which is lower than the asparaginases from E.coli and Erwinia. Essay -- DNA isolation and amplification of ansA1 and ansA3 genesGood quality genomic DNA was isolated (Sambrook, et al 2002) and bothansA1 and ansA3 genes were amplified by PCR. Clear bands of both the genes showing a size of 1kb were observed under UV transilluminator after agarose gel electrophoresis (F ig 1).Figure 1 PCR amplification of both ansA1 and ansA3 genes Lane (A) Step up 100bp marker, Lane (B) amplification of ansA1 gene, Lane (C) amplification of ansA3 geneOverexpression and purificationThe overexpressed recombinant proteins (rBliAI and rBliAIII) of B. licheniformis MTCC 429 were purified to homogeneity by affinity chromatography using Ni-NTA column. Overexpression of recombinant His-tagged asparaginase into E.coli leads to improvement in yield and affinity purification process of enzyme (Enriquez et al, 2012). The fractions showing presence of protein with the help of Bradfords reagent were pooled together. The pooled protein solution was dialyzed against the same buffer and was checked for the L-asparaginase activity.The purified protein after SDS PAGE or Native PAGE showed a single band (Fig 2) illustrating its homogeneous nature. The molecular weight of the subunits of ansA1 and ansA3was found to be approximately 37kD after SDS PAGE analysis. SDS PAGE analysis of re combinant L-asparaginase from Pyrococcus furiosus displayed single 37kDa band (Bansal et al, 2010). In another study, recombinant ansA from Rhizobium etliin SDS-PAGE showed the presence of a single polypeptide chain of 47kDa (Enriquez et al, 2012).Native PAGE analysis showed the molecular weight of the purified protein as 74 kDa. This study confirmed that the ansA1 and ansA3 enzymes from Bacillus licheniformis MTCC 429 are a homodimer in... ...81.50x106Roth et al, 2013Table Comparative kinetic parameters for hydrolysis of L-asparagineThe kinetic properties of recombinant BliA were investigated and the parameters like kcat and kcat/Km were also studied with L-asparagine as a substrate. Comparative studies of the rBliA with asparaginases from various microbial sources showed that rBliA has lower Km value for L-asparagine (Table 2) confirming thatrBliA hasbetter affinity towards its substrate than other reported (Table) microbial sources. The catalytic constant (kcat) of rBliA was on ly 1.5 times higher than the L-asparaginase from E.coli and catalytic constant of rErAII was 1.35 times higher than rBliA. The absolute value of kcat/Km suggests the catalytic efficiency of the enzyme (Price and Nairn, 2009). kcat/Km of the rBliAIII was 5.4x 104 which is lower than the asparaginases from E.coli and Erwinia.